
handle: 11000/33067
Introducción. Los trabajadores de la salud corren el riesgo de sufrir infecciones ocupacionales y se sabe que algunos procedimientos, como las broncoscopias, aumentan este riesgo. Por lo tanto, en este estudio se pretende valorar la carga y composición microbiana de los aerosoles generados por los pacientes durante la práctica de broncoscopias para cuantificar el riesgo ocupacional de los profesionales. Metodología. Se tomaron muestras de aerosoles durante la práctica rutinaria de broncoscopias en dos hospitales mediante muestreadores personales colocados en la ropa del profesional. Las muestras se cultivaron en distintos medios de cultivo, y se realizó la detección de virus respiratorios (SARS-CoV-2, Influenza A/B y VSR) mediante RT-PCR. Resultados. Se recogieron 29 muestras de las cuales se han obtenido un total de 234 aislamientos que corresponden 207 a especies bacterianas y 27 a especies fúngicas. Los géneros bacterianos más abundantes son Micrococcus, Staphylococcus y Bacillus y los fúngicos son Aspergillus, Talaromyces, Cladosporium y Trichoderma. También se detectaron un total de 8 muestras positivas para SARS-CoV-2. Conclusiones. Las bacterias cultivables de la microbiota oral, nasal y pulmonar se aerosolizan durante la broncoscopia y podrían ser inhaladas por el personal médico. La posible presencia de patógenos en esos aerosoles podría representar un riesgo de infección ocupacional.
Introduction. Healthcare workers are at risk of occupational infections and some procedures, such as bronchoscopies, are known to increase this risk. Therefore, this study aims to assess the microbial load and composition of aerosols generated by patients during bronchoscopy in order to quantify the occupational risk of professionals. Methodology. Aerosol samples were collected during routine bronchoscopy practice in two hospitals using personal samplers placed on the practitioner's clothing. Samples were cultured in different culture media, and detection of respiratory viruses (SARS-CoV-2, Influenza A/B and RSV) was performed by RT-PCR. Results. Twenty-nine samples were collected from which a total of 234 isolates were obtained, 207 of which correspond to bacterial species and 27 to fungal species. The most abundant bacterial genera are Micrococcus, Staphylococcus and Bacillus and the fungal genera are Aspergillus, Talaromyces, Cladosporium and Trichoderma. A total of 8 SARS-CoV-2 positive samples were also detected. Conclusions. Culturable bacteria from the oral, nasal and lung microbiota are aerosolised during bronchoscopy and could be inhaled by medical staff. The possible presence of pathogens in these aerosols could represent a risk of occupational infection.
bioaerosols, exposición ocupacional, patógenos, Bronchoscopy, microbiota, bioaerosoles, pathogens, occupational exposure, CDU::5 - Ciencias puras y naturales::57 - Biología, Broncoscopia
bioaerosols, exposición ocupacional, patógenos, Bronchoscopy, microbiota, bioaerosoles, pathogens, occupational exposure, CDU::5 - Ciencias puras y naturales::57 - Biología, Broncoscopia
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