A role for alpha-and beta-catenins in bacterial uptake.

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2000 France English Publisher:CCSDJournal:Proceedings of the National Academy of Sciences of the United States of America, volume 97 (issn: 0027-8424,

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Authors: Lecuit, Marc; Hurme, Reini; Pizarro-Cerdá, Javier; Ohayon, Hélène; Geiger, Benjamin; Cossart, Pascale;

pmid: 10963665

pmc: PMC27655

A role for alpha-and beta-catenins in bacterial uptake.

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Abstract

Interaction of internalin with E-cadherin promotes entry of Listeria monocytogenes into human epithelial cells. This process requires actin cytoskeleton rearrangements. Here we show, by using a series of stably transfected cell lines expressing E-cadherin variants, that the ectodomain of E-cadherin is sufficient for bacterial adherence and that the intracytoplasmic domain is required for entry. The critical cytoplasmic region was further mapped to the beta-catenin binding domain. Because beta-catenin is known to interact with alpha-catenin, which binds to actin, we generated a fusion molecule consisting of the ectodomain of E-cadherin and the actin binding site of alpha-catenin. Cells expressing this chimera were as permissive as E-cadherin-expressing cells. In agreement with these data, alpha- and beta-catenins as well as E-cadherin clustered and colocalized at the entry site, where F-actin then accumulated. Taken together, these results reveal that E-cadherin, via beta- and alpha-catenins, can trigger dynamic events of actin polymerization and membrane extensions culminating in bacterial uptake.

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France

Related Organizations

Département Sciences sociales, agriculture et alimentation, espace et environnement
France
Institut Pasteur
France
Inserm
France
Weizmann Institute of Science
Israel
Institut National de la Santé et la Recherche Médicale
France

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Keywords

MESH: Cytoskeletal Proteins, MESH: Trans-Activators, MESH: alpha Catenin, Listeria, [SDV]Life Sciences [q-bio], Recombinant Fusion Proteins, MESH: beta Catenin, 610, MESH: Actins, MESH: Listeria monocytogenes, Transfection, MESH: Cadherins, Cell Line, MESH: Listeria, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Recombinant Fusion Proteins, Tumor Cells, Cultured, Animals, Humans, MESH: Animals, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, beta Catenin, Sequence Deletion, Cultured, MESH: Humans, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, MESH: Transfection, MESH: Tumor Cells, MESH: Sequence Deletion, Cadherins, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Listeria monocytogenes, Actins, MESH: Cell Line, [SDV] Life Sciences [q-bio], Cytoskeletal Proteins, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Trans-Activators, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, alpha Catenin

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