
handle: 10953.1/18016
Este trabajo de fin de grado se ha realizado una búsqueda bibliográfica sobre los métodos de cuantificación existentes actualmente para fitohormonas y reguladores de crecimiento en muestras vegetales. Este trabajo consta de una breve introducción para poner en contexto que son las fitohormonas y los reguladores del crecimiento vegetal y que función ejercen cada uno de ellos en distintas zonas del organismo. Por dicha razón, es importante conocer la fisiología vegetal para saber en qué punto exacto realizan sus funciones, explicándose ésta detalladamente. Por otro lado, se detallará el tratamiento de muestra de cada fitohormona, generalmente basado en una extracción empleando como disolvente acetonitrilo. Posteriormente, se explicará en detalle los métodos analíticos existentes para su determinación, siendo los métodos basados en cromatografía líquida y cromatografía de gases con detectores UV y espectrometría de masas lo métodos más usados.
In this end-of-degree project, a short overview of existing quantification methods for the determination of phytohormones and growth regulators in plant samples has been carried out. This work consists of a brief introduction to contextualise what phytohormones and plant growth regulators are and what function each performs in different body areas. For this reason, it is crucial to know the plant physiology to know at what point strictly they perform their functions, explaining this in detail. On the other hand, the sample treatment of each phytohormone will be detailed, generally based on an extraction using acetonitrile as extraction solvent. Subsequently, the current analytical methods for its determination will be deeply explained. The most commonly employed are based on liquid chromatography and gas chromatography with UV detectors and mass spectrometry.
2301, Mass spectrometry, Chromatographic analysis, Química Analítica, Espectroscopía de masas, 2301.03, 2301.10, Analytical Chemistry, Análisis cromatográfico
2301, Mass spectrometry, Chromatographic analysis, Química Analítica, Espectroscopía de masas, 2301.03, 2301.10, Analytical Chemistry, Análisis cromatográfico
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