Autogeny was observed in a laboratory colony of Culex pipiens quinquefasciatus Say originating from Hyderabad, India. In a strain selected for an autogeny for 15 generations, all females, both mated and unmated, were able to mature eggs although the unmated females were unable to oviposit them. Ingesting glucose had little effect on the expression of autogeny and on the number of eggs in rafts in the laboratory experiments. Most autogenous females can ingest blood during first gonotrophic cycle, if the host is available. The blood feeding that occurred during the first cycle to initiate the second cycle was not confirmed, however, we cannot exclude this possibility.