Fluorogenic substrates for assay of chymosin.
Copyright policy )Fluorogenic substrates for assay of chymosin.
The use of fluorogenic substrates with intramolecular fluorescence quenching as substrates for chymosin was studied. It was shown that chymosin hydrolyzes the Phe-Phe peptide bond. The effect of pH on the hydrolysis of substrates by chymosin was investigated. The catalytic characteristics of the hydrolysis of the fluorogenic substrates were obtained at the pH optima. The influence of dimethylformamide on chymosin activity was studied.
- Lomonosov Moscow State University Russian Federation
Kinetics, Hydrolysis, Animals, Cattle, Dimethylformamide, Amino Acid Sequence, Hydrogen-Ion Concentration, Chymosin, Fluorescent Dyes, Substrate Specificity
Kinetics, Hydrolysis, Animals, Cattle, Dimethylformamide, Amino Acid Sequence, Hydrogen-Ion Concentration, Chymosin, Fluorescent Dyes, Substrate Specificity
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).2 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!