Flavonoids were produced from cell suspension cultures of Saussurea medusa. The results of studies on eight types of culture media showed that the MS medium was the best for cell growth and flavonoids formation, We investigated the effects of all the components of MS medium on the cell growth and flavonoids production, and found that carbon, nitrogen and phytohormone had especially marked effects. With MG medium a modified MS medium, the yield of cell growth was 24.8 g(dwt)/L, with MP medium another modified MS mediums, the yield of flavonoids production was 1. 75 g/L. The yield of cell growth and flavonoids production in MG and MP medium were 32% and 70% higher than that in MS medium respectively.