Mutations at position A960 of E. coli 23 S ribosomal RNA influence the structure of 5 S ribosomal RNA and the peptidyltransferase region of 23 S ribosomal RNA.
Copyright policy )Mutations at position A960 of E. coli 23 S ribosomal RNA influence the structure of 5 S ribosomal RNA and the peptidyltransferase region of 23 S ribosomal RNA.
The proximity of loop D of 5 S rRNA to two regions of 23 S rRNA, domain II involved in translocation and domain V involved in peptide bond formation, is known from previous cross-linking experiments. Here, we have used site-directed mutagenesis and chemical probing to further define these contacts and possible sites of communication between 5 S and 23 S rRNA. Three different mutants were constructed at position A960, a highly conserved nucleotide in domain II previously crosslinked to 5 S rRNA, and the mutant rRNAs were expressed from plasmids as homogeneous populations of ribosomes in Escherichia coli deficient in all seven chromosomal copies of the rRNA operon. Mutations A960U, A960G and, particularly, A960C caused structural rearrangements in the loop D of 5 S rRNA and in the peptidyltransferase region of domain V, as well as in the 960 loop itself. These observations support the proposal that loop D of 5 S rRNA participates in signal transmission between the ribosome centers responsible for peptide bond formation and translocation.
- Lomonosov Moscow State University Russian Federation
Aldehydes, Binding Sites, Base Sequence, Molecular Sequence Data, RNA, Ribosomal, 5S, RNA-Binding Proteins, CME-Carbodiimide, Butanones, GTP Phosphohydrolases, RNA, Bacterial, RNA, Ribosomal, 23S, Phenotype, RNA, Transfer, Genes, Bacterial, Protein Biosynthesis, Mutation, Peptidyl Transferases, Escherichia coli, Nucleic Acid Conformation, Ribosomes
Aldehydes, Binding Sites, Base Sequence, Molecular Sequence Data, RNA, Ribosomal, 5S, RNA-Binding Proteins, CME-Carbodiimide, Butanones, GTP Phosphohydrolases, RNA, Bacterial, RNA, Ribosomal, 23S, Phenotype, RNA, Transfer, Genes, Bacterial, Protein Biosynthesis, Mutation, Peptidyl Transferases, Escherichia coli, Nucleic Acid Conformation, Ribosomes
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