The omega-3 fatty acids Maresin 1 and Maresin 2 and the protein Annexin A1 are pro-resolving mediators which play an active role in resolution of inflammation. The mediators enhance elimination of apoptotic cells, activation of immune cells and limit invasion of leukocytes. The precursor of Maresin 1 and Maresin 2, the omega-3 fatty acid DHA, has been detected in tear fluid in individuals with chronic inflammation, indicating a role in resolution of ocular surface inflammation. The aims of this thesis were to investigate the intracellular pathways used by Maresin 1, Maresin 2 and Annexin A1 in rat conjunctival goblet cells and to examine the effect of the mediators on mucin secretion. Furthermore, the influence of the mediators on the allergy associated compound histamine was investigated. Rat conjunctival goblet cells were cultured. A ratio-imaging system using Fura-2/AM was used to measure changes in the peak of intracellular calcium in cytosol. An enzyme-linked lectin assay was used to measure changes in mucin secretion. In both calcium and mucin secretion experiments, the goblet cells were preincubated with inhibitors of different intracellular pathways before stimulation with either Maresin 1, Maresin 2 or Annexin A1. PCR was performed to explore if Annexin A1 was present in rat conjunctival goblet cells. We found all the mediators to increase intracellular calcium levels in rat conjunctival goblet cells by activation of different receptors and intracellular pathways. Moreover, we found Annexin A1 to be present in rat conjunctival goblet cells. Furthermore, preincubation with the mediators blocked the effect of histamine, indicating a potential role as a future treatment in allergic conjunctivitis. We believe that these mediators stimulate ocular surface homeostasis, both in health and disease.