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Recolector de Ciencia Abierta, RECOLECTA
Doctoral thesis . 2024
License: CC BY NC ND
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Impacto del ADN mitocondrial en la arquitectura de los genomas vegetales

Authors: Turón Ruiz, Aída;

Impacto del ADN mitocondrial en la arquitectura de los genomas vegetales

Abstract

En las últimas décadas se ha evidenciado la existencia de una transferencia de ADN mitocondrial al genoma nuclear en células vegetales. Este tránsito de material genético puede dar lugar al establecimiento de nuevos genes, o regiones génicas, y funciones génicas representando una fuente de evolución de las especies vegetales. Sin embargo, los mecanismos moleculares que se encuentran detrás de estos eventos de transferencia actualmente son desconocidos, siendo asociados comúnmente con la transferencia y reordenamiento primordial de genes durante el establecimiento de la endosimbiosis entre bacterias aeróbicas y células eucariotas ancestrales que dio lugar a las mitocondrias, pero sin indicio real de relación entre ambas. En este estudio se realizaron diferentes aproximaciones de genómica comparativa con el fin de dilucidar los factores implicados y las diferencias en las diversas especies vegetales de la transferencia de ADN mitocondrial al genoma nuclear. En primer lugar, se seleccionaron 16 especies representativas de diferentes linajes y se corroboró la existencia de material genético mitocondrial en sus genomas nucleares. Seguidamente, se seleccionaron diferentes parámetros asociados a los genomas los cuales sirvieron para realizar análisis comparativos con los que no fue posible encontrar patrones universales para todas las especies vegetales, pero sí sugerir la reformulación de premisas iniciales que, hoy en día y debido a la falta de investigaciones al respecto, continúan vigentes. Además, se pudo demostrar la concentración de los eventos de transferencia en momentos concretos del tiempo evolutivo, resultados que arrojan luz sobre el impacto real que puede tener el ADN mitocondrial en la evolución de la arquitectura y función de los genomas nucleares. Over the last decades, the existence of a transfer of mitochondrial DNA to the nuclear genome in plant cells has been evidenced. This transfer of genetic material can lead to the establishment of new genes, or gene regions, and gene functions representing a mechanism of evolution of plant species. However, the molecular mechanisms behind these transfer events are currently unknown, being commonly associated with primordial gene transfer and rearrangement during the establishment of endosymbiosis between aerobic bacteria and ancestral eukaryotic cells that gave rise to mitochondria, but with no real indication of a relationship between the two. In this study, different comparative genomics approaches were performed in order to elucidate the factors involved and the differences in the various plant species in the transfer of mitochondrial DNA to the nuclear genome. First, 16 representative species from different lineages were selected and the existence of mitochondrial genetic material in their nuclear genomes was corroborated. Next, different parameters associated with the genomes were selected and used to perform comparative analyses with which it was not possible to find universal patterns for all plant species, but it was possible to suggest the reformulation of initial premises that, nowadays and due to the lack of research in this regard, continue to be valid. In addition, it was possible to demonstrate the concentration of transfer events at specific moments in evolutionary time, results that shed light on the real impact that mitochondrial DNA can have on the evolution of the architecture and function of nuclear genomes.

Country
Spain
Related Organizations
Keywords

DNA transfer, Nuevos genes, Novel genes, Células vegetales, Comparative genomics, Nuclear genomes, Genómica comparativa, Transferencia de ADN, ADN mitocondrial, Plant cells, Genomas nucleares, Mitochondrial DNA

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green