[Use of RT-PCR and nested PCR for diagnosing enterovirus infections].
Copyright policy )[Use of RT-PCR and nested PCR for diagnosing enterovirus infections].
An application of reverse transcription (RT-PCR) and nested-PCR for the detection of human enteroviruses isolated in Poland is described. A specific 297-bp fragment was amplified by the method from all studied enteroviruses strains. Amplification system and the nested primers allowed to detect RNA isolated from 10(1) TCID50/ml which corresponded to one molecule of viral RNA in tested volume. However, applicability of the method for routine diagnosis of enterovirus neuro-infection should be reassessed in extensive clinical studies.
Reverse Transcriptase Polymerase Chain Reaction, Enterovirus Infections, Humans, RNA, Viral, Polymerase Chain Reaction, Enterovirus
Reverse Transcriptase Polymerase Chain Reaction, Enterovirus Infections, Humans, RNA, Viral, Polymerase Chain Reaction, Enterovirus
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