
O potencial biotecnológico dos microrganismos extremófilos tem vindo a merecer uma atenção cada vez maior da comunidade científica internacional. Onde antes este organismos eram vistos como meras curiosidades, começam agora a ser considerados soluções viáveis para os problemas económicos e/ou ecológicos de diversos processos industriais, não só devido aos seus metabolismos muito extremos, mas devido às próprias enzimas que produzem, as quais possuem muitas vezes propriedades com enorme potencial biotecnológico. A obtenção por parte do ICAT de uma colecção de estirpes procariotas provenientes de fontes hidrotermais da Crista Média Atlântica no Mar dos Açores consistiu numa oportunidade valiosa para a descoberta de novas enzimas com possíveis usos industriais. No presente trabalho, faz-se um resumo do conhecimento referente aos microrganismos extremófilos e às suas aplicações na indústria e, numa segunda parte, inicia-se um processo de ensaio e selecção de uma colecção de 140 estirpes procariotas na tentativa de descobrir novas enzimas com potencial biotecnológico. ABSTRACT: The biotechnological potential of extremophile microorganisms has been getting the increasing attention of the international scientific community. Whereas these organisms used to be seen as simple curiosities, they are now being considered as viable solutions to the economical and/or ecological problems associated with various industrial processes, not only because of their extreme metabolisms, but also because of their enzymes, whose properties often have tremendous biotechnological potential. A collection of prokaryotic strains from hydrothermal springs along the Mid- Atlantic Ridge in the Azores Sea was obtained by ICAT, and provided a valuable opportunity for the discovery of new enzymes with potential industrial applications. In this work, the present knowledge on extremophile microorganisms and their applications in various industries is summarized and, in a second part, a process of screening and selection of a collection of 140 prokaryotic strains in initiated, in the attempt to find new enzymes with biotechnological potential.
Mestrado em Microbiologia Molecular
Microorganismos, Enzimas, Microbiologia molecular, Biotecnologia
Microorganismos, Enzimas, Microbiologia molecular, Biotecnologia
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