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Bioensaios ecotoxicológicos com microcrustáceos: Daphnia magna

Authors: Ramos, Sofia;

Bioensaios ecotoxicológicos com microcrustáceos: Daphnia magna

Abstract

This work was developed under the scope of EPIBOOST project, focusing on the freshwater micro crustacean Daphnia magna and the toxic effect of cadmium, allying the study of the ecotoxicity of an emergent pollutant, with the context of climate change and predicted global warming. Hence, the main goal of this work was to compare the sensitivity of D. magna to cadmium at three different temperatures, 15°C, 20°C and 25°C. The cultures were maintained in controlled conditions of temperature (15°C, 20°C and 25°C) and photoperiod (16L:8N), using ASTM medium, Ascophyllum nodosum extract and Raphidocellis subcapitata as a food source. A fixed range of seven concentrations of cadmium (2.1 mg/L – 238 mg/L) was tested under the three temperatures according to the OECD guideline for Daphnia sp. acute immobilization test. The temperature showed to influence the sensitivity to cadmium. Higher temperatures increase the sensitivity to this metal, resulting in a diminishing of the EC values (15º C – no immobilization recorded; 20º C - EC50 = 32.294 mg/L; and 25º C - EC50 = 2.130 mg/L). This work contributed to showing the negative influence of global warming on a model organism with an essential role in the health of the ecosystem.

Este trabalho foi desenvolvido no âmbito do projeto EPIBOOST e teve como objectivo principal estudar o efeito tóxico do cádmio no microcrustáceo de água doce Daphnia magna, num contexto de alterações climáticas. Assim, o principal objetivo deste trabalho foi comparar a sensibilidade de D. magna a cádmio, em três temperaturas diferentes, 15°C, 20°C e 25°C. As culturas foram mantidas em condições controladas de temperatura (15°C, 20°C e 25°C) e fotoperíodo (16L:8N), utilizando meio ASTM, extrato de Ascophyllum nodosum e Raphidocellis subcapitata como fonte de alimento. Foi testada uma gama fixa de sete concentrações de cádmio (2,1 mg/L - 238 mg/L) nas três temperaturas, de acordo com a diretriz da OECD para o teste de imobilização aguda de Daphnia sp. De acordo com os resultados, a temperatura mostrou influenciar a sensibilidade dos organismos a este poluente. Temperaturas mais elevadas aumentaram a sensibilidade ao metal, resultando numa diminuição dos valores de EC (15º C - não se registou imobilização; 20º C - EC50 = 32.294 mg/L; e 25º C - EC50 = 2.130 mg/L). Este trabalho contribuiu para demostrar a influência negativa do aquecimento global num organismo modelo, com um papel essencial na saúde do ecossistema.

Not Published

Country
Portugal
Related Organizations
Keywords

Toxicidade aguda, Daphnia magna, Cádmio, Temperatura

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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