
handle: 10722/181105
OBJECTIVES: To use a multi-gene sequencing approach to characterize genetic diversity within T. putidum strains, to establish their phylogenetic relationships with other species of human and non-human treponemes. METHODS: Sets of primers based on T. denticola gene sequences, were screened for their ability to PCR amplify gene fragments from five T. putidum strains (OMZ 758 (type), 844, 846, 847, 848) as well as several dog and human T. denticola isolates (generously supplied by Dr. Chris Wyss). Individual and concatenated gene sequences were analyzed using various computational evolutionary biology approaches, including Bayesian inference and Maximum Likelihood (ML) ...
Poster Session: Scientific Groups - 88. Microbiology/Immunology II: no. 691
postprint
Nucleic acids, Periodontal organisms, Molecular biology, Microbial phylogeny, Microbiology
Nucleic acids, Periodontal organisms, Molecular biology, Microbial phylogeny, Microbiology
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