Identification of serotypes of Rickettsia tsutsugamushi by nested polymerase chain reaction.

G J, Shieh; H L, Chen; H Y, Chen; G R, Wang; C B, Horng

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Article . 1999

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Identification of serotypes of Rickettsia tsutsugamushi by nested polymerase chain reaction.

descriptionPublicationkeyboard_double_arrow_right Article 22 Dec 1999 English Journal:Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology, volume 29 (issn: 0253-2662,

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Authors: G J, Shieh; H L, Chen; H Y, Chen; G R, Wang; C B, Horng;

pmid: 10592793

Identification of serotypes of Rickettsia tsutsugamushi by nested polymerase chain reaction.

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Abstract

Nested polymerase chain reaction (PCR) was applied to identify the serotypes of Rickettsia tsutsugamushi isolated from patients. The primers used for PCR were based on the nucleotide sequences encoding a 56 kDa antigen of rickettsiae. Comparing to the conventional immunofluorescence assay (IFA), which displays a considerable degree of cross-reactivity among different species, the result obtained suggests that the polymerase chain reaction method is much more reliable than IFA.

Keywords

Orientia tsutsugamushi, Guinea Pigs, Animals, Fluorescent Antibody Technique, Humans, Serotyping, Polymerase Chain Reaction

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