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Matrices nanocompuestas de alcohol de polivinilo (PVA)/celulosa bacteriana (CB) para el crecimiento celular y la regeneración de tejidos

Authors: Restrepo Múnera, Luz Marina; Martínez, Lina; Agudelo Vélez , Camilo Andrés; Zuluaga Gallego, Robin; Velásquez Cock, Jorge Andrés; Gañán Rojo, Piedad Felisinda; Castro Herazo, Cristina Isabel; +2 Authors

Matrices nanocompuestas de alcohol de polivinilo (PVA)/celulosa bacteriana (CB) para el crecimiento celular y la regeneración de tejidos

Abstract

RESUMEN: En esta investigación se desarrollaron biomateriales de PVA, CB y nanocompuestos PVA/CB mediante fermentación in situ utilizando una bacteria productora de celulosa Gluconacetobacter medellinensis. Los biomateriales fueron caracterizados mediante microscopía electrónica de barrido (SEM), pruebas de hinchamiento y proliferación celular in-vitro utilizando células de piel humana (Fibroblastos). No se observaron cambios en la morfología celular por el uso de los biomateriales y su microestructura estuvo estrechamente ligada a la proliferación celular. Las células mostraron una mayor afinidad por la CB, resultado relacionado con su similitud en morfología y estructura química con el colágeno. La respuesta obtenida sumada a la capacidad de la CB de imitar la forma de la interfaz gas-líquido durante su síntesis, permitió la construcción de una prenda apósito de CB en forma de guante que podría tener aplicaciones en el tratamiento de quemaduras y úlceras cutáneas.

ABSTRACT: In this research, biomaterials of PVA, CB and in situ fermented PVA/CB nanocomposites were developed, using a cellulose producer bacterium, Gluconacetobacter medellinensis. The biomaterials were characterized through scanning electron microscopy (SEM), swelling and in vitro cell proliferation assay with skin cells (Fibroblast). Changes in the cell morphology were not found by the use of the biomaterials and the cell proliferation is associated with the microstructure of them. Cells showed a higher affinity to CB, outcome related to the morphological and chemical similitudes with collagen. Joining this response with the CB capacity of mimic the gas-liquid interface, was developed a glove shaped wound dressing that will have applications in burn and skin ulcer treatment.

Country
Colombia
Keywords

Crecimiento celular, Rrenda apósito, Material nanocompuesto, 610, Celulosa bacteriana, Alcohol polivinílico

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green