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Evaluación de pretratamientos químicos para la hidrólisis enzimática de residuos lignocelulósicos de yuca (Manihot esculenta Crantz)

Authors: Niño López, Lilibeth Caridad; Acosta Cárdenas, Alejandro; Gelves Zambrano, Germán Ricardo;

Evaluación de pretratamientos químicos para la hidrólisis enzimática de residuos lignocelulósicos de yuca (Manihot esculenta Crantz)

Abstract

RESUMEN: El efecto de diferentes pretratamientos químicos con ácido sulfúrico (H2SO4), hidróxido de sodio (NaOH) y peróxido de hidrogeno (H2O2), fueron evaluados mediante la cuantificación de azucares reductores totales producidos durante la etapa de hidrólisis enzimática de los residuos lignocelulósicos de yuca (Manihot esculenta Crantz) y el porcentaje (%) de remoción de lignina después del pretratamiento. La cantidad de residuos utilizados (1 y 5%) (w/v) a diferentes tamaños de partícula (1,18 y 0,6mm) fueron pretratados por separado (hojas y tallos) con H2SO4, NaOH y H2O2 a diferentes concentraciones (1 y 5%) (w/v). Los residuos vegetales fueron hidrolizados con la enzima accellerase 1500 a 50°C, pH 5 y 140 rpm. El pretratamiento con NaOH permite la mayor liberación de azucares reductores totales durante la hidrólisis enzimática (3,7g/L) en hojas seguido del pretratamiento con H2SO4 (2,11g/L) y H2O2 (1,54g/L). En tallos las concentraciones de azúcares son menores.

ABSTRACT: The effect of different chemical pretreatment with sulfuric acid (H2SO4), sodium hydroxide (NaOH) and hydrogen peroxide (H2O2) were evaluated by quantifying total reducing sugars produced during enzymatic hydrolysis step of lignocellulosic residues cassava (Manihot esculenta Crantz) and lignin removal percentage after pretreatment. The amount of waste used (1 to 5%) (w/v) in different particle sizes (1.18 and 0.6 mm) were pretreated separately (leaves and stems) with H2SO4, NaOH and H2O2 at varying concentrations (1 and 5%) (w/v). Plant residues were hydrolyzed with enzyme Accellerase 1500 with 50 ° C, pH 5 and 140 rpm. NaOH pretreatment enables higher total reducing sugars released during enzymatic hydrolysis (3.7 g/L) followed by leave pretreatments using H2SO4 (2.11 g/L) and H2O2 (1.54 g/L). Sugar concentrations were lower in stem pretreatments.

COL0066991

Country
Colombia
Keywords

550, etanol, 610, lignin, Hidrólisis enzimática, celulosa, Pretratamientos químicos, pretratamiento, Yuca: Manihot esculenta, hemicellulose, Crantz, lignocellulosic waste, lignocelulósicos, Engineering (General). Civil engineering (General), cellulose, hidrólisis, lignina, hydrolysis, Yuca: Manihot esculenta, Crantz;, residuos lignocelulósicos, Pretratamiento, hemicelulosa, ethanol, TA1-2040, Pretreatment

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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