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Article . 2008
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DELIGNIFICACIÓN SELECTIVA DEL PASTO Pennisetum purpureum x Pennisetum typhoides USANDO BASIDIOMICETOS LIGNINOLÍTICOS SELECTIVE DELIGNIFICATION OF THE Pennisetum purpureum x Pennisetum typhoides GRASS USING LIGNINOLYTIC BASIDIOMYCETES

Authors: Segura Sánchez, Freimar; Echeverri Flórez, Rosario; Mejía Gallón, Amanda Inés;

DELIGNIFICACIÓN SELECTIVA DEL PASTO Pennisetum purpureum x Pennisetum typhoides USANDO BASIDIOMICETOS LIGNINOLÍTICOS SELECTIVE DELIGNIFICATION OF THE Pennisetum purpureum x Pennisetum typhoides GRASS USING LIGNINOLYTIC BASIDIOMYCETES

Abstract

RESUMEN: Muestras de pasto Pennisetum purpureum x Pennisetum typhoides de 120 días de corte se someten durante siete semanas a fermentación selectiva en estado sólido (FES) con cepas de Ganoderma ssp y Lentinus ssp. Se realiza la caracterización de la delignificación por Infrarrojo con Transformada de Fourier (IR-TF) midiendo las áreas de las principales bandas características. Mediante esta técnica se establece que en las muestras tratadas con Ganoderma spp se obtiene una pérdida del 70% de los compuestos aromáticos con relación a los alifáticos. En las semanas cero y séptima se establecieron valores de lignina en detergente ácido (LDA) de 55,9% y 10,7%, respectivamente. En este mismo período los contenidos de materia seca y celulosa variaron del 73,3% al 92,9% y del 3,1% al 51,7% respectivamente. Estos resultados confirman una degradación selectiva de la lignina en las muestras tratadas con Ganoderma spp y medios suplementados con manganeso. Las pruebas de degradabilidad in situ, utilizando la técnica de la bolsa de nailon y de digestibilidad in vitro de la materia seca basada en técnicas enzimáticas y gravimétricas, no mostraron mejoramiento de la digestibilidad de la materia seca del pasto como consecuencia de la fermentación con las dos cepas de hongos basidiomicetos, corroborando lo indicado por otros autores que afirman que los hongos pueden ser tóxicos para la microflora del rumen y, por lo tanto, pueden afectar la digestibilidad de la materia seca.

ABSTRACT: Samples of Pennisetum purpureum x Pennisetum typhoides grass of 120 days are put under selective fermentation in solid state with two strains of basidiomycetes fungi Ganoderma ssp and Lentinus ssp, during seven weeks. The delignification characterization is made by Fourier Transform Infrared Spectroscopy (FTIR) measuring the areas of the main characteristic bands. In the samples treated with the Ganoderma ssp strain, a loss of 70% of aromatic compounds in relation to the aliphatics is obtained (by FTIR); also are obtained in the zero and seventh weeks, the acid-detergent lignin (ADL) (55,9% and 10,7%, respectively) as well as the contents of dry matter and cellulose that vary from the 73,3% to the 92,9% and from the 3,1% to the 51,7% respectively between the zero and seventh weeks, results that confirm an selective degradation of the lignin. The digestibility tests in vitro, that are in according with the in situ test, do not evidence an improvement in the grass digestibility by the ruminants. As this is degraded by the Ganoderma ssp strain is not obtained an increase of the digestibility of the dry matter, corroborating the ideas explained for other authors who affirm that the ingestion of fungi can be toxic for the rumen’s microflora, and therefore to be toxic for the animals also.

COL0065152

Country
Colombia
Keywords

Basidiomycetes, FTIR analysis of Pennisetum purpureum x Pennisetum typhoides Grass, degradabilidad in situ, in vitro digestibility, Pennisetum purpureum x Pennisetum typhoides Grass, fermentación en estado sólido, digestibilidad in vitro, TP368-456, 540, Lignin, 630, Food processing and manufacture, Pennisetum purpureum, Solid state fermentation, http://aims.fao.org/aos/agrovoc/c_5677, http://aims.fao.org/aos/agrovoc/c_13199, pasto Pennisetum purpureum x Pennisetum typhoides, delignificación selectiva, Basidiomicetos, Selective delignification, basidiomicetos, Pennisetum glaucum, HD9665-9675, Pharmaceutical industry

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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