[The transactivation of hepatitis B virus X protein on the expression of Fas ligand in HepG2 cells].
Copyright policy )[The transactivation of hepatitis B virus X protein on the expression of Fas ligand in HepG2 cells].
To study the effect of hepatitis B virus x (HBx) protein on the expression of Fas Ligand in HepG2 cells.The open reading frame of HBx gene was obtained from HBV genome (subtype adw) digested with Nco I and Bgl II, then cloned into pCEP4. The reconstructive plasmid pCEP4x was transfected into HepG2 cells by electroportion method. After selecting with hygromycin B, resistant clonies were obtained. The expressions of HBx gene and Fas Ligand gene were detected by RT-PCR. Immunohistochemical staining and western blot analysis.It showed that Fas Ligand gene expressed in the modified HepG2 cells transfected with plasmid pCEP4x.The results suggest the expression of Fas Ligand in HepG2 cells could be activated by HBx protein.
- Chongqing Medical University China (People's Republic of)
Fas Ligand Protein, Membrane Glycoproteins, Gene Expression Regulation, Liver, Trans-Activators, Humans, Viral Regulatory and Accessory Proteins, Transfection, Cell Line
Fas Ligand Protein, Membrane Glycoproteins, Gene Expression Regulation, Liver, Trans-Activators, Humans, Viral Regulatory and Accessory Proteins, Transfection, Cell Line
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