The aim of this study was to investigate the effects that two different training programs have on the characteristics of the accelerations (ACC) and decelerations (DCC) of the soccer players and their ability to maintain them over time in a training task. Twenty-three female soccer players from a Spanish professional club were split into three groups: a Small-sided game training (SGG), an eccentric overload training (EOG) and a control group (CG). The ACC and DCC variables were analysed in a training task before and after the training protocol using GPS devices. The SSG improved the total distance travelled [ES (CI) = 0.32 (0.04; 0.60)], the number of ACC >2 m/s2 [ES (CI) = 0.60 (-0.16; 1.36)], distance travelling ACC between 2-3 m/s2 [ES (CI) = 0.62 (-0.01; 1.25)], initial velocity ACC [ES (CI) = 0.88 (-0.10; 1.86)] and the percentage of repeated ACC [ES (IC) = 0.58 (-0.11; 1.28)] with respect to the CG. The EOG group improved distance travelling ACC [ES (CI) = 0.66 (-0.10; 1.41)] and DCC [ES (CI) = 1.21 (0.77; 1.64)] over 3 m/s2, maximum ACC [ES (CI) = 0.92 (0.40; 1.44)] and DCC [ES (CI) = 1.16 (0.58; 1.74)], average ACC [ES (CI) = 1.07 (0.40; 1.74)] and DCC [ES (CI) = 0.91 (0.24; 1.54)] and the number of DCC >2 m/s2 [ES (CI) = 0.54 (-0.18; 1.26)] with respect to the CG. In conclusion, the SGG obtained improvements in the ACC variables, while the EOG brought about improvements in the ACC and DCC variables.