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Estudo in vitro da resistência adesiva à dentina cariada e análise morfológica da interface

Authors: Martins, Ana Margarida Henriques;

Estudo in vitro da resistência adesiva à dentina cariada e análise morfológica da interface

Abstract

Objetivo: Avaliar se o substrato (dentina cariada vs. dentina saudável) tem influência na morfologia da interface adesiva e na resistência adesiva à microtração às 24 horas do adesivo All-Bond Universal®, no modo etch&rinse, sujeito a pressão pulpar. Materiais e Métodos: 40 molares humanos foram testados, encontrando-se divididos em 2 grupos: Grupo A – Dentes com lesão de cárie envolvendo dentina; Grupo B – Dentes hígidos (controlo). Após aplicação do sistema adesivo e restauração em resina composta, os espécimes foram armazenados a 37 ºC durante 24 horas, sendo posteriormente cortados de modo a obter palitos para o estudo da resistência adesiva, sendo 3 espécimes de cada grupo direcionados para a observação em microscopia eletrónica de varrimento (MEV). Os resultados do teste de microtração foram analisados segundo os testes Shapiro-Wilk e Levene, não sendo garantidos os prossupostos de normalidade e homogeneidade, utilizando-se para comparação entre grupos o teste não paramétrico de Mann-Whitney. Resultados: Relativamente à análise da resistência adesiva, verificaram-se diferenças estatisticamente significativas entre substratos (p<0,05), sendo os valores dos espécimes do Grupo B mais elevados. Na análise das interfaces adesivas em MEV, foram também detetadas diferenças, exibindo os espécimes do Grupo A uma camada híbrida de fina ou mesmo indetetável, com prolongamentos de resina de pequenas dimensões. Já nos espécimes do Grupo B, a camada híbrida observada é mais espessa e os prolongamentos resinosos de grandes comprimentos, possuindo até prolongamentos acessórios. Conclusões: Os valores de resistência adesiva após 24 horas foram mais elevados nos espécimes do Grupo B. Verificaram-se diferenças na interface adesiva consoante o substrato dentinário. São necessários mais estudos para comprovar que a performance em dentina cariada do sistema adesivo estudado é, de facto, pior do que em dentina saudável.

Objectives: To evaluate whether the substrate (healthy vs. carious dentin) has influence on the morphology of the adhesive interface and on the 24 hours microtensile bond strengths promoted by the adhesive system All-Bond Universal®, in etch&rinse mode and under pulpal pressure. Materials and Methods: 40 human molars were tested, divided into 2 groups: Group A – Teeth with a single carious lesion involving dentine; Group B – Healthy teeth (control). After adhesive procedures and consequent composite restoration, the specimens were stored for 24 hours in an incubator, and were then cut to be submitted to microtensile bond strength testing as well as scanning electron microscope (SEM) observation. The microtensile bond strength results were analyzed by Shapiro-Wilk and Levene, not guaranteeing the normality and homogeneity assumptions. Therefore, the comparison of the results was made with non-parametric Mann-Whitney test. Results: Microtensile bond strength differences between the experimental groups were detected (p<0,05), with higher values being recorded on the specimens from Group B. Concerning the SEM observation, there were differences between groups as well: specimens from Group A had a thin hybrid layer and many small length resin tags were present; In specimens from Group B, the hybrid layer was thicker, containing many and long resin tags, with accessory extensions. Conclusions: There were differences in the interface depending on the substrate were the adhesive was applied. The 24 hours microtensile bond strengths to healthy dentin were significantly higher than those to carious dentin. Further studies are required to prove that the adhesive system studied has in fact a worst performance in carious dentine.

Tese de mestrado, Medicina Dentária, Universidade de Lisboa, Faculdade de Medicina Dentária, 2018

Country
Portugal
Related Organizations
Keywords

Teses de mestrado - 2018, Domínio/Área Científica::Ciências Médicas::Medicina Clínica, Adesivos dentários, Materiais dentários

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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