Constitutive expression of VEGF, VEGFR-1, and VEGFR-2 in normal eyes.
Copyright policy )Constitutive expression of VEGF, VEGFR-1, and VEGFR-2 in normal eyes.
The expression of vascular endothelial growth factor (VEGF) and its high-affinity receptors VEGFR-1 and VEGFR-2 was investigated in normal eyes.Monkey and rat eyes were surgically removed in animals under deep anesthesia and immediately prepared for study. Ocular VEGF, VEGFR-1, and VEGFR-2 expression was studied using a combination of in situ hybridization, northern blot analysis, immunohistochemistry, immunoassay, and reverse transcription-polymerase chain reaction.Steady state VEGF mRNA levels were detected in the normal vascularized ocular tissues of the monkey: the conjunctiva, iris, retina, and the choroid-retinal pigment epithelial complex. VEGF121 and VEGF165 were the major isoforms identified. VEGF protein was detected in the conjunctiva, retina, and the choroid-retinal pigment epithelial complex. Retinal VEGF mRNA localized to the ganglion, inner nuclear, and retinal pigment epithelial cell layers. VEGF protein localized to these same layers and to the cones of monkey retina. VEGFR-1 and VEGFR-2 mRNAs were detected in normal monkey iris, retina, and the choroid-retinal pigment epithelial complex. In both monkey and rat eyes, VEGFR-1 and VEGFR-2 mRNAs were localized to the inner nuclear layer of the retina.VEGF, VEGFR-1, and VEGFR-2 are constitutively expressed in the vascularized tissues of normal eyes.
- Harvard University United States
Male, Lymphokines, Gene Expression, Receptor Protein-Tyrosine Kinases, Enzyme-Linked Immunosorbent Assay, Endothelial Growth Factors, Blotting, Northern, Eye, Macaca mulatta, Rats, Immunoenzyme Techniques, Rats, Sprague-Dawley, Macaca fascicularis, Receptors, Vascular Endothelial Growth Factor, Proto-Oncogene Proteins, Animals, Receptors, Growth Factor, RNA, Messenger, In Situ Hybridization, DNA Primers
Male, Lymphokines, Gene Expression, Receptor Protein-Tyrosine Kinases, Enzyme-Linked Immunosorbent Assay, Endothelial Growth Factors, Blotting, Northern, Eye, Macaca mulatta, Rats, Immunoenzyme Techniques, Rats, Sprague-Dawley, Macaca fascicularis, Receptors, Vascular Endothelial Growth Factor, Proto-Oncogene Proteins, Animals, Receptors, Growth Factor, RNA, Messenger, In Situ Hybridization, DNA Primers
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).184 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!