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Master thesis . 2021
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Vantagens da adição de antioxidantes ao diluidor para conservação de sémen de suíno

Authors: Félix, Maria Inês Pires;

Vantagens da adição de antioxidantes ao diluidor para conservação de sémen de suíno

Abstract

Os suínos são animais extremamente sensíveis ao stress térmico, tendo as altas temperaturas efeitos negativos sobre os parâmetros reprodutivos das fêmeas e a qualidade do sémen dos machos. Esta influência negativa sobre os parâmetros espermáticos, assim como a diminuição da qualidade das doses de sémen durante o armazenamento, deve-se em parte às espécies reativas de oxigénio, que quando em excesso causam stress oxidativo e têm uma série de consequências, nomeadamente a diminuição da motilidade dos espermatozóides e a instabilização das membranas lipídicas e do ADN, o que consequentemente afeta o poder fertilizante dos espermatozóides. A relação entre a qualidade do sémen e os resultados de fertilidade é um indicador importante para as explorações e centros de inseminação. Neste contexto, o presente ensaio teve como objetivo avaliar o efeito da utilização de um diluidor suplementado com antioxidantes (MR-A® antiox), em comparação com um diluidor comercial (MR-A®), nos parâmetros de fertilidade in vivo, nomeadamente na taxa de gestação, taxa de parto, número de leitões vivos (NV) e número de leitões mortos (NM), e ainda estimar a influência da temperatura sobre os mesmos. Para o efeito, foram inseminadas 134 porcas F1 (LW x LR), com sémen proveniente do mesmo varrasco RAM2 (Duroc x Pietrain), divididas em dois grupos: grupo controlo (GC) com o diluidor comercial MR-A ® e grupo teste (GT) com o diluidor MR-A® antiox. Procedeu- se ainda a uma breve análise do sémen, e posteriormente foram registados para cada porca o diagnóstico de gestação, os NV, os NM e os mumificados. Os resultados não mostraram diferenças significativas (p>0,05) entre os grupos, pelo que o desempenho das fêmeas inseminadas não foi influenciado pelo diluidor utilizado, embora as médias no GC tenham sido superiores em todos os parâmetros analisados (taxa de gestação de GC 9,5% superior ao GT; NV = 11.79 vs 11.04; NM = 2.14 vs 2.17). Foi também possível aferir a influência da paridade sobre a prolificidade (p<0,05), sendo que fêmeas com maior número de partos apresentaram menor número de NV e maior número de NM. Por outro lado, também foi registada a influência da temperatura sobre a taxa de gestação, concluindo-se que quanto mais baixas as temperaturas maior o número de diagnósticos positivos.

ABSTRACT - Advantages of adding antioxidants to swine semen extender for conservation - Pigs are extremely susceptible to thermal stress, with high temperatures having negative effects on reproductive parameters of females and semen quality of males. This negative influence on espermatic parameters, as well as the decrease in the quality of semen doses during storage, is partly due to reactive oxygen species, which when in excess cause oxidative stress and have a series of consequences, namely the reduction of sperm motility and instabilization of lipid membranes and DNA, which consequently affects the fertilizing power of sperm. The relationship between semen quality and fertility results is an important indicator for farms and insemination centers. In this context, the present assay aims to evaluate the effect of using an extender supplemented with antioxidants (MR-A ® antiox), in comparison with a commercial extender (MR-A®), in the parameters of fertility in vivo, namely in the gestation rate, farrowing rate, number of piglets born alive (NV) and number of stillborns (NM), and also estimate de influence of temperature on them. For this purpose, 134 sows F1 (LW x LR) were inseminated, with semen from the same boar RAM2 (Duroc x Pietrain), divided in two groups: control group (GC) with the commercial MR-A® extender and test group (GT) with MR-A® antiox extender. A brief analysis of semen was also carried out, and subsequently the gestation diagnosis, NV, NM and mummified were recorded for each sow. The results did not show any significant diferences (p>0,05) between groups, which means that the performance of the inseminated sows was not influenced by the extender, although the averages in the GC were higher in all analysed parameters (gestation rate of GC was 9,5% above GT; NV = 11.79 vs 11.04; NM = 2.14 vs 2.17). It was also possible to assess the influence of parity on litter size (p<0,05), where females with higher number of births show lower number of NV and higher number of NM. On the other hand, the influence of temperature on gestation rate were also recorded, concluding that the lower the temperature, the higher the number of positive diagnos

Dissertação de Mestrado Integrado em Medicina Veterinária

N/A

Country
Portugal
Keywords

reactive oxygen species, fertility, espécies reativas de oxigénio, semen diluents, varrasco, boar, Antioxidantes, fertilidade, diluidores de sémen, Antioxidants

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selected citations
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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