This thesis proposal is about the application of ligninolytic enzymes and reactive species of Mn3+ in the degradation of recalcitrant compounds, including dyes, pharmaceuticals and personal care products, and endocrine disrupting compounds. These compounds have a polymeric structure which is hard to degrade; therefore, it is necessary to use degradation agents with high oxidizing potential. These studies were conducted in laboratory assays in order to maximize the degradation of these compounds. Subsequently, application arises in enzyme reactors, evaluating the parameters that ensure high operational efficiency and processing capacity.