The proceedings contain 44 papers. The topics discussed include: time-resolved nanoscale imaging of biomolecules in living cells and tissues: prospects for small-animal imaging; spectrally resolved fluorescence lifetime and FRET measurements; three-dimensional FRET microscopy; improving fluorescence diagnosis of cancer by SLIM; scanning total internal reflection fluorescence imaging; two-photon spectral imaging microscopy of skin tissues; CARS microscopy for the monitoring of fat deposition mechanisms in a living organism; cumulant analysis in two-color fluorescence fluctuation spectroscopy; fluorescence lifetime imaging of human skin and hair; and photonic crystal fiber as a tunable light source for visible wavelength two-photon microscopy.