<script type="text/javascript">
<!--
document.write('<div id="oa_widget"></div>');
document.write('<script type="text/javascript" src="https://www.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=undefined&type=result"></script>');
-->
</script>

COPY SCRIPT

For further information contact us at helpdesk@openaire.eu

Enhanced lateral flow immunoassay using gold nanoparticles loaded with enzymes

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 01 Feb 2013 Spain English Publisher:Elsevier BVJournal:Biosensors and Bioelectronics, volume 40, pages 412-416 (issn: 0956-5663,

Authors: Parolo, Claudio; Escosura-Muñiz, Alfredo de la; Merkoçi, Arben;

doi: 10.1016/j.bios.2012.06.049

pmid: 22795532

handle: 10261/99307

Enhanced lateral flow immunoassay using gold nanoparticles loaded with enzymes

- Summary
- Subjects
- Metrics

Abstract

The use of gold nanoparticles (AuNPs) as labeling carriers in combination with the enzymatic activity of the horseradish peroxidase (HRP) in order to achieve an improved optical lateral flow immunoassay (LFIA) performance is presented here. Briefly in a LFIA with an immune-sandwich format AuNPs are functionalized with a detection antibody already modified with HRP, obtaining an 'enhanced' label. Two different detection strategies have been tested: the first one following just the red color of the AuNPs and the second one using a substrate for the HRP (3 different substrates are evaluated), which produces a darker color that enhances the intensity of the previous red color of the unmodified AuNPs. In such very simple way it is gaining sensitivity (up to 1 order of magnitude) without losing the simplicity of the LFIA format, opening the way to other LFIA applications including their on-demand performance tuning according to the analytical scenario.

Country

Spain

Related Organizations

Keywords

Immunoassay, Protein Array Analysis, Metal Nanoparticles, Reproducibility of Results, Biosensing Techniques, Equipment Design, Microfluidic Analytical Techniques, Sensitivity and Specificity, Equipment Failure Analysis, Coated Materials, Biocompatible, Gold, Horseradish Peroxidase

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	292
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 1%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 1%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 1%