Development of serological tests to detect anti-GFP antibodies for differentiating Brucella infected and GFP-vaccinated sheep

Authors: Zabalza-Baranguá, Ana; San Román, Beatriz; Muñoz, Pilar M.; Chacón Diaz, Carlos; Moreno Robles, Eduardo; Blasco, José M.; Grilló, María Jesús;

handle: 10261/98751

Development of serological tests to detect anti-GFP antibodies for differentiating Brucella infected and GFP-vaccinated sheep

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Abstract

Serological interference in diagnostic tests is a major problem associated to the use of veterinary vaccines, which hamper the differentiation of infected and vaccinated animals (DIVA). To solve this problem in the context of brucellosis, our group is developing new Brucella vaccines tagged with green fluorescent protein (GFP) as xenogenic marker to be used in combination with associated DIVA diagnostic tests. The aim of this work was to develop Gel Diffusion (GD-GFP) and indirect-ELISA (ELISA-GFP) assays able to detect specifically anti-GFP antibodies in sheep.

Póster presentado en el 5th Congress of European Microbiologists, celebrado en Leipzig (Alemania) del 21 al 25 de julio de 2013.

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