Telomeres, which are found at the ends of eukaryotic chromosomes, are composed of tandem arrays of re- petitive sequences and safeguard genomic stability. Previous studies have revealed that telomeric repeats are also present at internal chromosomal loci in many eukaryotes. However, the biological role of these in- terstitial telomeric sequences (ITSs) remains un- known. The integrity of telomeric length and chro- matin structure is required for telomere stability. However, the study of these telomeric features can be impeded by the presence of ITSs. Frequently cutting restriction enzymes have been revealed to be very useful tools for the study of the length and chromatin structure of telomeres independent of the presence of ITSs.

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