Expression of a yeast gene can be blocked by insertion of short yeast DNA fragments between a UAS and the TATA box

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 1995 English Publisher:Springer Science and Business Media LLCJournal:Current Genetics, volume 27, pages 387-389 (issn: 0172-8083, eissn: 1432-0983,

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Authors: Vincent, Olivier; Gancedo, Juana M.;

doi: 10.1007/bf00352109

pmid: 7614563

handle: 10261/78969

Expression of a yeast gene can be blocked by insertion of short yeast DNA fragments between a UAS and the TATA box

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Abstract

We have constructed a plasmid, pOV10, which facilitates the introduction of putative upstream activating sequences (UAS) or upstream repressing sequences (URS) from yeast genes into plasmids containing CYCl-lacZ fusions. We have observed that the insertion of yeast sequences from 155 to 195 bp between the UAS and the TATA box of a CYCl-lacZ fusion gene can block beta-galactosidase expression. It is suggested that this block is related to the formation of nucleosomes on the DNA.

Keywords

Saccharomyces cerevisiae Proteins, Genetic Vectors, Cytochromes c, Cytochrome c Group, Saccharomyces cerevisiae, Regulatory Sequences, Nucleic Acid, beta-Galactosidase, TATA Box, Gene Expression Regulation, Fungal, Cloning, Molecular, Plasmids

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