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handle: 10261/76481
[EN] The invention relates to a recombinant nucIeotide sequence that incIudes the sequence of the mrfp gene that codes for a red autoíluorescent protein optimized for expression in lactic bacteria, as well as the vectors that comprise same. The invention likewise relates to the cells that incIude the sequence or the vector of the invention and the uses thereof. In addition, the invention relates to the use of both the sequence and the vector for the detection and characterization of a unidirectional or bidirectional promoter region, as well as to the use thereof for transcription. The invention further relates to the use of cells that contain the sequence or vector of the invention, for detennining the colonizing ability of cells. The invention also relates to the methods associated with the uses of the invention, to a kit that incIudes the elements of the invention and to the use thereof.
[ES] La presente invención se refiere a una secuencia nucIeotidica recombinante que comprende la secuencia del gen mrfp que codifica para una proteina autoíluorescente roja optimizada para la expresión en bacterias lácticas asi como a los vectores que la comprenden. La invención también se refiere a las células que comprenden la secuencia o el vector de la invención y sus usos. Asi mismo, también se refiere al uso tanto de la secuencia como del vector para la detección y caracterización de una región promotora unidireccional o bidireccional, asi como de su uso para transcripción. Además, se refiere al uso de las células que contienen la secuencia de la invención o el vector de la invención para la determinación de la capacidad colonizadora de células. También se refiere a los métodos relacionados con los usos de la invención, a un kit que comprende los elementos de la invención y su uso.
A1 Solicitud de patente con informe sobre el estado de la técnica
Consejo Superior de Investigaciones Científicas
Peer reviewed
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