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TDX (Tesis Doctorals en Xarxa)
Doctoral thesis . 2013
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Doctoral thesis . 2013
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Analysis of the efficiency in the Spanish National Barley Breeding Program. Past results and prospects for future improvements using molecular markers

Authors: Mansour Elsayed, Elsayed;

Analysis of the efficiency in the Spanish National Barley Breeding Program. Past results and prospects for future improvements using molecular markers

Abstract

[ES] Los objetivos generales de esta tesis fueron evaluar la eficiencia de la selección en el Programa Nacional Español de Mejora de Cebada y encontrar los factores genéticos responsables de la ventaja del material elite, para facilitar la selección en el futuro.El progreso en el programa se estimó retrospectivamente, usando datos generados en los ensayos de las generaciones avanzadas (F8, F9, F10) a lo largo de una serie de años representativa. La relación entre la GEI y variables climáticas se investigó con más detalle utilizando los datos retrospectivos de 11 años de generaciones avanzadas del programa de mejora, correspondientes a 12 localidades Se llevó a cabo un análisis de QTL con una población de líneas consanguíneas recombinantes (recombinant inbred lines, RIL), desarrollada a partir de uno de los mejores cruzamentos del programa de mejora. Algunos de los QTLs se detectaron se proponen como futuros objetivos de mejora para usar la selección asistida por marcadores (MAS) en los mismos tipos de cruzamentos. Un estudio de QTLs de selección en las líneas avanzadas del programa confirmó la gran influencia de la región de VrnH1 en el proceso de mejora. Los QTLs de selección detectados en las líneas avanzadas del programa confirmaron sólo algunos de los QTL encontrados en la población RIL pero, por otro lado, proporcionaron evidencia de la presencia de una fuerte selección en varias regiones genómicas, que también pueden ser objetivo de MAS en el futuro.

[CA] Els objectius generals d'aquesta tesi foren avaluar l'eficiència de la selecció al Programa Nacional Espanyol de Millora d¿Ordi i trobar els factors genètics responsables de l'avantatge del material elit, per facilitar la selecció al futur. El progrés en el programa es va estimar retrospectivament, utilitzant dades generades als assajos de les generacions avançades (F8, F9, F10) al llarg d'una sèrie d'anys representativa. La relació entre la GEH i variables climàtiques es va investigar amb més detall utilitzant les dades retrospectives de 11 anys de generacions avançades del programa de millora, corresponents a 12 localitats. Es va dur a terme una anàlisi de QTL amb una població de línies consanguínies recombinants (recombinant inbred lines, RIL), desenvolupada a partir d'un dels millors creuaments del programa de millora. Alguns d'ells es proposen com a futurs objectius de millora per utilitzar la selecció assistida per marcadors (MAS) als mateixos tipus de creuaments. Un estudi de QTLs de selecció en les línies avançades del programa va confirmar la gran influència de la regió de VrnH1 en el procés de millora. Els QTLs de selecció detectats en les línies avançades del programa van confirmar només alguns dels QTL trobats a la població RIL però, d'altra banda, van proporcionar evidència de la presència d'una forta selecció en diverses regions genòmiques, que també poden ser objectiu de MAS en el futur.

[EN] The overall objectives of this thesis were to test the efficiency of selection in the Spanish Barley Breeding Program and to find the most important genetic factors responsible for the advantage of elite material, to facilitate future selection. The progress in the breeding program was estimated restrospectively, using data generated trials of the advanced generations (F8, F9, F10) over a long series of years. The relationship between GEI and climatic variables was investigated in more detail using again retrospective data of 11 years of advanced generations of the breeding program, across 12 locations. Quantitative trait loci analysis (QTLs) was done for a population of recombinant inbred lines (RILs) developed from one of the best crosses of the breeding program. Some of the QTLs detected are proposed as future breeding targets for marker assisted selection (MAS) in the same type of crosses. A study of selection QTLs in the advanced lines of the program confirmed the large influence of the region VrnH1 in the breeding process. Only a few selection QTLs confirmed the QTLs found in the RIL population but, on the other hand, provided evidence for strong selection at several genomic regions that may be targeted through MAS in the future.

I would like to the Spanish Ministry of Science and Innovation (MICINN) for supporting this work (scholarship BES-2008-009623, projects AGL2007-63625 and AGL2010-21929).

210 Pags., with Tabls. and Figs.

Peer reviewed

Country
Spain
Keywords

QTL, Barley, Ordi, Mejora genética vegetal, Molecular markers, Millora genètica, 633, Marcadores moleculares, Genética, Marcadors moleculars, Cebada, Plant breeding

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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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