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Método de detección y cuantificación de las razas patogénicas del hongo Fusarium oxysporum f. sp. ciceris

Authors: Landa, Blanca B.; Montes Borrego, Miguel; Navas Cortés, Juan Antonio; Jiménez-Díaz, Rafael M.; Jiménez Fernández, Daniel;

Método de detección y cuantificación de las razas patogénicas del hongo Fusarium oxysporum f. sp. ciceris

Abstract

La presente invención proporciona un método, basado en PCR convencional o cuantitativa en tiempo real (qPCR), fiable, rápido, exacto y reproducible para la detección y cuantificación de cualesquiera de las ocho razas patogénicas del hongo Fusarium oxysporum f. sp. ciceris (0, 1A, 1B/C, 2, 3, 4, 5 ó 6) en una muestra biológica aislada. Dicho método permite la detección y cuantificación de, como mínimo, 1 pg de ADN del patógeno en muestras biológicas complejas incluyendo diferentes tejidos de la planta de garbanzo y suelos infestados, sin que su exactitud y eficacia se vea alterada. La invención también se refiere a dos parejas de cebadores útiles para llevar a cabo el método de la invención.

Consejo Superior de Investigaciones Científicas (España), Universidad de Córdoba

A1 Solicitud de patente con informe sobre el estado de la técnica

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
views
OpenAIRE UsageCountsViews provided by UsageCounts
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