Relaxation of chromatin structure upon removal of histones H2A and H2B

descriptionPublicationkeyboard_double_arrow_right Article 25 Jun 1984 Spain English Publisher:WileyJournal:FEBS Letters, volume 172, pages 70-74 (issn: 0014-5793, eissn: 1873-3468,

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Authors: Jordano, Juan; Montero, F.; Palacián, Enrique;

doi: 10.1016/0014-5793(84)80876-5

pmid: 6734822

handle: 10261/65692

Relaxation of chromatin structure upon removal of histones H2A and H2B

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Abstract

Modification of chromatin from chicken erythrocytes with dimethylmaleic anhydride is accompanied by its solubilization and the dissociation of histones H1, H5, H2A and H2B. Histone H1 is the first to dissociate and H5 the last. After regeneration of the modified amino groups, residual chromatin preparations with different histone composition were studied by circular dichroism and thermal denaturation. In addition to the effects produced by the lack of histones HI and H5, both techniques show a substantial relaxation of chromatin structure induced by the loss of histones H2A and H2B, which appear to play an important role in the superhelical folding of DNA.

Country

Spain

Related Organizations

Complutense University of Madrid
Spain
Spanish National Research Council
Spain

Keywords

Erythrocytes, Hot Temperature, Circular Dichroism, DNA, Chromatin structure Histone H2A Histone H2B Histone dissociation DNA folding Dimethylmaleic anhydride, Chromatin, Histones, Solubility, Animals, Nucleic Acid Conformation, Chickens, Maleic Anhydrides

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