Protein G-horseradish peroxidase based method for light-microscope immunocytochemistry. Application to the pituitary gland of the killifish, Fundulus heteroclitus.

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1994 English Publisher:Società Italiana di IstochimicaJournal:European journal of histochemistry : EJH, volume 38 (issn: 1121-760X,

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Authors: García-García, A.; Muñoz-Cueto, José Antonio; Rodríguez Martínez, Ramón B.; Sarasquete, Carmen;

pmid: 7530516

handle: 10261/6327

Protein G-horseradish peroxidase based method for light-microscope immunocytochemistry. Application to the pituitary gland of the killifish, Fundulus heteroclitus.

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Abstract

Horseradish peroxidase-protein G conjugate was used to localize anti-human luteinizing hormone and anti-human chorionic gonadotropin primary antibodies bound to gonadotropins in paraffin and Historesin embedded sections of Fundulus heteroclitus pituitaries. The sensitivity and specificity of this method and those obtained after the avidin-biotin-peroxidase complex, the streptavidin-biotin-peroxidase complex and the peroxidase-antiperoxidase procedures were compared. The protein G-horseradish peroxidase method gave a clear immunostaining of gonadotrophic cells with virtually no background. Detection efficiency was reduced in comparison with the other techniques. As for the embedding medium, hydrophilic resin Historesin considerably enhanced the structural detail and the resolution of the immunostaining at light-microscope level.

8 pages.-- PMID: 7530516.

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Keywords

Killifishes, Nerve Tissue Proteins, Luteinizing Hormone, Chorionic Gonadotropin, Sensitivity and Specificity, Immunoenzyme Techniques, Immunoglobulin G, Pituitary Gland, Animals, Horseradish, Light microscopy, Gonadotrophic cells, Immunocytochemistry, Horseradish Peroxidase

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