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DIGITAL.CSIC
Conference object . 2012 . Peer-reviewed
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Desarrollo de un ensayo de eclosión de huevos para detectar resistencia antihelmíntical al albendazol en cepas ovinas de Fasciola hepática

Authors: Robles Pérez, D.; Martínez Pérez, José Manuel; Valcárcel Sancho, Félix María; Rojo Vázquez, Francisco Antonio; Martínez Valladares, María;

Desarrollo de un ensayo de eclosión de huevos para detectar resistencia antihelmíntical al albendazol en cepas ovinas de Fasciola hepática

Abstract

[ES] El objetivo de este estudio, fue desarrollar un ensayo de eclosión de huevos (EHA) para detectar cepas ovinas de Fasciolahepatica, sensibles y resistentes al albendazol (ABZ). Para la cepa sensible, se observaron diferencias significativas entre las concentraciones más altas (2, 0,2 y 0,02μg/ml)y un control sin fármaco, a diferencia de lacepa resistente. De esta forma se confirmó el efecto del ABZ en la cepa sensible. También se confirmó la resistencia con huevos recogidos a los siete días del tratamiento de animales infectados con una cepa resistente. Sin embargo, a los 15 días posttratamiento (p.t.) la RA se diluyó debido al efecto de la reinfección. Esto es debido a que en el día 7 p.t. sólo hay fasciolas adultas y el día 15 p.t. coexisten adultos e inmaduros, comportándose como un conjunto con tendencia a la susceptibilidad en cuanto al EHA.

[EN] In this study, an egg hatch assay (EHA) was developedwith faecaleggs for the detection of the resistance of some isolates of Fasciola hepatica, susceptible and resistant to albendazole (ABZ). For the susceptible isolate, significant differences between the three highest concentrations (2, 0.2 and 0.02 μg/ml) and the control well have been found, but not for the resistant isolate, confirming the effect of this drug in the susceptible isolate. Also the resistance was confirmed with eggs obtained 7 days after the treatment of animals infected with a resistant isolate. Nevertheless, to 15 days post - treatment (pt) the degree of RA waslower diluted because of the presence of both resistant adult flukes and immature when the drug was administered.

Este estudio ha sido financiado por los proyectos nacionales del INIA-MEC, RTA 2006-00183-C03-02 y RTA2010-00094-C03-02. M. Martínez-Valladares disfruta de un contrato post-doctorado Jae-Doc del CSIC co-financiado por el Fondo Social Europeo.

5 páginas, 2 figuras.-- Trabajo presentado al XXXVII congreso de la SEOC (Ciudad Real, España, 19 al 21 de septiembre del 2012).

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Country
Spain
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Keywords

EHA, Resistencia antihelmíntica, Fasciola hepatica, Albendazole, Anthelmintic resistance, Albendazol

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selected citations
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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