Reversed-phase high-performance liquid chromatographic separation of mono- and divinyl chlorophyll forms using pyridine-containing mobile phases and a polymeric octadecylsilica column

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1997 English Publisher:Springer Science and Business Media LLCJournal:Chromatographia, volume 44, pages 43-49 (issn: 0009-5893, eissn: 1612-1112,

Copyright policy )

Authors: Garrido, J. L.; Zapata, Manuel;

doi: 10.1007/bf02466514

handle: 10261/58968

Reversed-phase high-performance liquid chromatographic separation of mono- and divinyl chlorophyll forms using pyridine-containing mobile phases and a polymeric octadecylsilica column

- Summary
- Subjects
- Metrics

Abstract

The separation of chlorophyll forms was studied employing a wide bore polymeric octadecylsilica column and pyridine containing mobile phases, giving consideration to considering the influence of mobile phase composition and column temperature on the resolution of monovinyl forms from their divinyl analogues. A method involving gradient elution and operating at 15 ~ is proposed for the separation of several polar and non-polar mono- and divinyl chlorophylls from etiolated tissues of higher plants and from marine phytoplankton. The advantages of pyridine as a mobile phase additive in the reversed-phase liquid chromatography of chlorophylls are discussed

7 pages, 8 figures

Peer reviewed

Related Organizations

Spanish National Research Council
Spain
Xunta de Galicia
Spain
Instituto de Investigaciones Marinas
Spain

Keywords

Mono- and divinyl chlorophylls, Pyridine containing mobile phases, Column liquid chromatography, Polymeric ODS phases

Impact byBIP!

	selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	17
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average