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handle: 10261/47165
[ES] En el presente trabajo se han realizado estudios virológicos en distintos lotes de quistes comerciales de artemias utilizados como alimento de larvas de peces marinos en piscifactorías. Mediante nested-PCR se ha detectado genoma del virus de linfocistis (LCDV) en homogeneizados de quistes y nauplios de artemia. La existencia de partículas víricas infectivas en estos homogeneizados se ha demostrado mediante la aparición de efectos citopáticos en cultivos celulares. Los ensayos de hibridación in situ han demostrado la existencia del LCDV en artemias infectadas naturalmente, así como en artemias inoculadas mediante baño, sin observarse alteraciones morfológicas. Las artemias actúan por tanto como bioacumuladores, pudiendo desempeñar un papel importante como reservorios ambientales de patógenos de peces. Estos resultados ponen de manifiesto el riesgo potencial de las artemias como fuente de patógenos víricos en estadios larvarios.
[EN] Cysts and naupliis of Artemia sp. were analysed. Specific nested-PCR revealed lymphocystis disease virus (LCDV) genome. Infective viral particle has been observed by CPE development in inoculated cell cultures. Viral genome was found by in situ hybridization in nauplii and adults of natural infected artemia, as in nauplii of bath challenged artemia. No morphological damages have been observed. Artemia is a bio-accumulator of fish pathogens, with a possible role as environmental reservoir of fish pathogens. These results have shown the risk of artemia as a source of viral pathogens to fish larvae.
Irene Cano es contratada postdoctoral en el ICMAN.CSIC dentro del Fondo social europeo- I3P-CSIC, en el marco del proyecto AGL2006-17777-C03-02/ACU (IP: Carmen Sarasquete).
4 páginas, 1 figura. XI Congreso Nacional de Acuicultura (Vigo, 24-28 septiembre 2007). Ed. Antonio Cerviño Eiroa, Alejandro Guerra Díaz y Carmen Pérez Acosta.
Peer reviewed
Lymphocystis disease virus (LCDV), Virus linfocistis (LCDV), Estadios larvarios, Piscifactorias, Artemia
Lymphocystis disease virus (LCDV), Virus linfocistis (LCDV), Estadios larvarios, Piscifactorias, Artemia
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