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Susceptibilidad a "Plasmopara viticola" (Berk. & Curt.) Berl. & de Toni, en diferentes variedades de vid ("Vitis vinifera" L.)

Authors: Boso Alonso, Susana; Santiago Blanco, José Luis; Alonso-Villaverde Iglesias, Virginia; Gago Montaña, Pilar; Martínez Rodríguez, María del Carmen;

Susceptibilidad a "Plasmopara viticola" (Berk. & Curt.) Berl. & de Toni, en diferentes variedades de vid ("Vitis vinifera" L.)

Abstract

[ES] El mildiu es una de las enfermedades de vid más destructivas a nivel mundial, y está causado por el oomycete Plasmopara viticola (Berk. & Curt.) Berl.& de Toni. El objetivo de este trabajo fue estudiar los niveles de susceptibilidad a P. viticola en algunas de las viníferas más utilizadas en diferentes viñedos europeos. Se estudiaron 5 variedades de Vitis vinifera L. conocidos como altamente susceptibles (cvs. Albariño, Tempranillo, Touriga Nacional, Riesling, y Cabernet Sauvignon), y Vitis rupestris, considerada como altamente resistente. Se inoculó P. viticola en laboratorio, utilizando dos metodologías distintas (Técnica del disco de la hoja y plantas). Los resultados obtenidos permitieron diferenciar tres grupos: uno formado por los poco susceptibles, en el que se incluían variedades como Cabernet Sauvignon y Riesling; un segundo grupo formado por los muy susceptibles, en el que se incluían el Albariño, Touriga Nacional y Tempranillo y un tercer grupo con el genotipo más resistente (V. rupestris). Además se describe el modo de colonización de P. viticola en estas variedades, tanto a nivel espacial como temporal, haciendo un seguimiento mediante microscopía de epifluorescencia, del curso de colonización del hongo por el mesófilo de la hoja, desde su penetración, hasta la formación de la vesícula subestomática y posterior formación de esporangióforos.

[EN] Downy mildew, caused by the obligate oomycete Plasmopara viticola (Berk. & Curt.) Berl. & de Toni., is one of the most destructive grapevine diseases occurring worldwide. The aim of the present study was to examine susceptibility to infection by P. viticola in different grapevine varieties grown in European vineyards. Five cultivars of V. vinifera L. known to be susceptible (Albariño, Tempranillo, Touriga Nacional, Riesling and Cabernet Sauvignon), and one Vitis genotypes known to be resistant (V. rupestris) were examined. Plants were inoculated with P. viticola in the laboratory using the leaf disc and plant techniques. The obtained results demonstrated, that there were 3 groups: one formed by those little susceptible, e.g., cvs. Cabernet Sauvignon and Riesling; and a second group formed by those very susceptible, cvs. Albariño, Touriga Nacional and Tempranillo, and a third group of more resistant genotype (V. rupestris). Furthermore, the course of colonization of leaf mesophyll by P. viticola was described in these varieties, both spatial and temporary level, making a monitoring through microscopic epifluorescence, from its penetration to the formation of the substomatic vesicle, and later formation sporangiophores.

Peer reviewed

Country
Spain
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Keywords

Esporulación, Resistencia, Sporulation, Resistance, Spp Vitis, Severidad, Incidencia, Disease severity, Disease incidence

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selected citations
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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