4D-STEM records a 2D electron diffraction pattern using a pixelated electron detector, while scanning the electron probe in 2D across the specimen. Thus it captures the full diffraction pattern from each scan location. This technology has demonstrated uses in enhancing STEM image quality (Seifer, Elbaum 2024, Varnevides 2024) and has the potential to be used for quantitative analysis of the sample composition (Seifer, Houben 2024). 4D-STEM has further been adapted in our lab for cryotomography of biological specimens (Seifer, Elbaum 2024). However, conventional tomographic reconstruction addresses only a single measured intensity rather than a 2D array. We have developed the Tomo4D pipeline to reconstruct a 2D diffraction pattern for each voxel (x,y,z) in the scanned sample. This provides depth resolution within the projected diffraction patterns. We demonstrate Tomo4D on a T4 Bacteriophage sample containing in addition gold nanoparticles for alignment, showing reconstructed diffraction patterns of both the disordered materials of the sample and the crystalline gold nanoparticles.

Resumen del trabajo presentado al 89th Annual Meeting of the Israel Chemical Society, celebrado los días 10 y 11 de febrero de 2026 en Tel Aviv.

No