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Histochemical and biochemical study of lipids during the reproductive cycle of the toadfish, Halobatrachus didactylus (Schneider, 1801).

Authors: Muñoz-Cueto, José Antonio; Álvarez, Marina; Blanco, Miguel; González de Canales, María Luisa; García-García, A.; Sarasquete, Carmen;

Histochemical and biochemical study of lipids during the reproductive cycle of the toadfish, Halobatrachus didactylus (Schneider, 1801).

Abstract

[ES] Se estudian aspectos histoquímicos y bioquímicos de lípidos durante el ciclo reproductivo anual de hembras de pez sapo, Halobatrachus didactylus. En el cityoplasma de los ovocitos vitelogénicos se han observado glóbulos lipídicos conteniendo lípidos neutros y gránulos proteicos constituidos por fosfolípidos y glicolípidos. Los lípidos neutros (gotas de grasa) también están presentes en el citoplasma de los hepatocitos. Las mayores concentraciones de lípidos se han observado en el hígado y las menores en el suero. Los triglicéridos fueron los lípidos predominantes en el hígado. La concentración de lípidos totales y fosfolípidos hepáticos y gonadales, así como los niveles de triglicéridos hepáticos han sido superiores durante el periodo reproductivo (de enero a mayo). Se han establecido coeficientes de correlación positivos entre el índice gonosómico (GSI) y hepatosómico (HSI) y la concentración de lípidos totales, colesterol y fosfolípidos gonadales. Mientras el HSI mostró una correlación positiva con los lípidos hepáticos y séricos, el GSI presentó una coeficiente de correlación negativo con los lípidos séricos. Estos resultados muestran que los cambios que experimentan el GSI y el HSI, a lo largo del ciclo reproductivo de hembras de H. didactylus, están aasociados con modificaciones histológico-histoquímicas y con variaciones en la concentración de lípidos ovários, hepáticos y séricos.

[EN] Histochemical and biochemical aspects of the lipids of the female toadfish, Halobatrachus didactyllus, during its annual reproductive cycle were studied. Oil droplets containing neutral lipids, and protein-yolk granules containing phospholipids and glycolipids were observed in the vitellogenic oocytes. The cytoplasm of the hepatocytes also contained neutral lipids (oil droplets). The highest lipid concentrations were observed in the liver and the lowest in the serum. In the liver, triglycerides predominated in the fractional composition of lipids. The ovarian and hepatic total lipids and phospholipids, and the hepatic triglyceride levels were higher during the reproductive period (from January to May). A positive relationship between the gonadosomatic index (GSI), hepatosomatic index (HSI) and ovarian total lipids, cholesterol, and phospholipids was found. The HSI was also positively correlated with the hepatic and serum lipids, whereas the GSI showed a negative relationship with the serum lipids. Results obtained show that the GSI and HSI variations observed, during the annual reproductive cycle of H. didactylus females, are associated with histological-histochemical changes and to modifications in the ovarian, hepatic and serum lipid concentrations.

8 páginas, 2 figuras, 2 tablas.

Peer reviewed

Keywords

Serum, Bioquímica, Toadfish, Suero, Histoquímica, Ciclo reproductivo, Hígado, Ovary, Biochemistry, Reproductive cycle, Liver, Ovario, Lípidos, Histochemistry, Pez sapo

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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