DNA methylation increases throughout Arabidopsis development

descriptionPublicationkeyboard_double_arrow_right Article 21 Jun 2005 Spain English Publisher:Springer Science and Business Media LLCJournal:Planta, volume 222, pages 301-306 (issn: 0032-0935, eissn: 1432-2048,

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Authors: Ruiz-García, L.; Cervera, M.T.; Martínez-Zapater, J.M.;

doi: 10.1007/s00425-005-1524-6

pmid: 15968513

handle: 10261/42339

DNA methylation increases throughout Arabidopsis development

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Abstract

We used amplified fragment length polymorphisms (AFLP) to analyze the stability of DNA methylation throughout Arabidopsis development. AFLP can detect genome-wide changes in cytosine methylation produced by DNA demethylation agents, such as 5-azacytidine, or specific mutations at the DDM1 locus. In both cases, cytosine demethylation is associated with a general increase in the presence of amplified fragments. Using this approach, we followed DNA methylation at methylation sensitive restriction sites throughout Arabidopsis development. The results show a progressive DNA methylation trend from cotyledons to vegetative organs to reproductive organs.

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Spain

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Keywords

5-azacytidine, DNA, Plant, Gene Expression Regulation, Plant, Plant development, Arabidopsis, DNA methylation pattern, Gene Expression Regulation, Developmental, ddm1 mutant, DNA Methylation

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