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DIGITAL.CSIC
Article . 2011 . Peer-reviewed
Data sources: DIGITAL.CSIC
Applied Physics Letters
Article . 2011 . Peer-reviewed
Data sources: Crossref
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Observation of spermidine-induced attractive forces in self-assembled monolayers of single stranded DNA using a microcantilever sensor

Authors: Mertens, Johann; Tamayo de Miguel, Francisco Javier; Kosaka, Priscila M.; Calleja, Montserrat;

Observation of spermidine-induced attractive forces in self-assembled monolayers of single stranded DNA using a microcantilever sensor

Abstract

Despite the biological relevance, the physical origin of attraction between highly negatively charged DNA strands in condensation remains an open question. We have used microcantilever sensors to study the forces involved in DNA condensation by spermidine. The experiments were performed under flow conditions with gold-coated cantilevers sensitized with thiolated single stranded DNA. The experiments show that above a critical concentration of spermidine, the DNA strands abruptly experiences large attractive forces. The critical spermidine concentration for the transition increases with the monovalent salt concentration. The experiments provide a direct insight of the forces responsible of condensation.

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Spain
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Keywords

Monolayers, Micromechanical devices, BioMEMS, DNA, Self-assembly, Molecular biophysics, Cantilevers, Coatings, Gold

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
views
OpenAIRE UsageCountsViews provided by UsageCounts
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12
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