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Micron
Article . 2025 . Peer-reviewed
License: CC BY NC
Data sources: Crossref
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DIGITAL.CSIC
Article . 2025 . Peer-reviewed
Data sources: DIGITAL.CSIC
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A histological procedure for the study of insect chitinized tissues in light microscopy

Authors: Gallardo, Manuela; Valladolid, María;

A histological procedure for the study of insect chitinized tissues in light microscopy

Abstract

We present a procedure to obtain histological preparations of insect cuticles for their observation in ligth microscopy. The use of EDTA (ethylendiaminetetraacetic acid), as a chelator of certain metals present in chitin, soften the tissues and allows us to cut them. The tissues were embbeding in plastic resines in two different protocols and cutted by cryostat (20-30 μm) and ultramicrotome (4 μm) respectively. This procedure, based on traditional histology products, allows us to obtain preparations observable under an optical microscope, facilitating the study of the internal structure of the cuticule, e.g. visualizing the different layers or structures such as glands or setae.

Countries
Spain, Spain
Related Organizations
Keywords

Microscopy, Integument, Cuticle, Insecta, Histological Techniques, EDTA, Animals, Chitin, Light microscopy, Microtomy, Insect, Edetic Acid

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
1
Average
Average
Average
Green
hybrid