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Producción de poli(3-hidroxibutirato-4-hidroxibutirato) mediante ingeniería metabólica en Azohydromonas lata DSM 1123

Authors: Chacón, Paula; Ibero, Juan; Sanz, Eugenio; Prieto, María Auxiliadora; Galán, Beatriz; García, José Luis;

Producción de poli(3-hidroxibutirato-4-hidroxibutirato) mediante ingeniería metabólica en Azohydromonas lata DSM 1123

Abstract

Los polihidroxialcanoatos (PHAs) son una familia de poliésteres bacterianos producidos por algunas bacterias en condiciones de desequilibrio nutricional. Dependiendo de su composición monomérica existen muchos tipos de PHAs, dando lugar a una gran variedad de materiales con propiedades diversas. Uno de los PHAs mejor estudiados es el poli-β-hidroxibutirato (PHB), formado por monómeros de cadena corta de 3-hidroxibutirato.Este material es, sin embargo, difícil de utilizar y procesar debido a sus propiedades térmicas y mecánicas. La incorporación de otros monómeros como el 4-hidroxibutirato (4HB) al polímero da lugar a la producción de copolímeros comercialmente más atractivos. Así, el poli(3-hidroxibutirato-4-hidroxibutirato) [P(3HB-4HB)] presenta propiedades interesantes debido a su elevada flexibilidad y menor cristalinidad. En el presente trabajo utilizamos la cepa productora de PHA Azohydromonas lata (anteriormente Alcaligenes latus), en la que se ha descrito la mayor productividad de acumulación de PHB hasta la fecha. Es una bacteria gram-negativa que se encuentra en diversos ambientes, como el suelo, agua de mar y entornos contaminados. Este microorganismo es capaz de fijar nitrógeno, crece en condiciones de aerobiosis y puede usar la sacarosa como fuente de carbono. Además, ésta producción está asociada al crecimiento y no requiere limitación de nutrientes.

Proyecto SynEco (TED2021-130689B-C32) financiado por MCIN/AEI/10.13039/501100011033 y NextGenerationEU/PRTR de la Unión Europea.

Peer reviewed

1 p.-5 fig.

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green