Intertidal mudflats are among the most productive coastal ecosystems, largely because of the activity of the photosynthetic microbial community on the sediment surface, known as microphytobenthos (MPB). While the dynamics of MPB have been extensively studied in temperate estuaries, there is limited research in tropical estuaries. To address this knowledge gap, we investigated the spatio-temporal dynamics of MPB in the Nicoya Gulf (Costa Rica), one of the world's most productive tropical estuaries, using Sentinel-2 images at 10 m spatial resolution from 2018 to 2022. We used Normalized Difference Vegetation Index (NDVI) values ranging from 0 to 0.4 to monitor MPB cover and growth rates (μNDVI, days-1). Analysis of data using Generalized Additive Models (GAM) showed that up to 62 % of the temporal variability in average MPB NDVI, peaking in late wet and early dry seasons (November-December), can be explained by years and months. The high temperatures and irradiance during the dry season (December to April) in the Nicoya estuary may inhibit MPB growth, contrasting with patterns observed in temperate estuaries. We observed higher MPB NDVI in the upper intertidal zone (mean sea level > 0.5 m) as usually occurs in temperate estuaries. This research highlights the importance of high-resolution satellite imagery for long-term monitoring of MPB dynamics in tropical tidal flats, offering a valuable tool for estimating ecosystem services provided by intertidal MPB, such as primary production, climate regulation, and nutrient cycling on a global scale.