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handle: 10261/371284
[ES] El presente estudio tuvo por objetivo comparar las lesiones histológicas de truchas arcoíris (Oncorhynchus mikyss) afectadas por la Enfermedad Bacteriana del Agua Fría (BCDW) con muestras de piel de truchas asintomáticas procedentes de centros piscícolas en el Lago Titicaca (Puno, Perú) e identificar la presencia de Flavobacterium psychrophilum mediante PCR convencional. Se trabajó con 13 truchas con lesiones de piel y siete sin lesiones, con tallas superiores a los 25 cm y peso promedio de 350 g. En el PCR para la identificación de F. psychrophilum se utilizaron iniciadores dirigidos a la región 16S ARNr. Molecularmente, ambos grupos de animales resultaron positivos para la presencia de F. psycrophilum. Se puede concluir que este patógeno está presente en la zona sur del país.
[EN] The aim of this study was to compare histological lesions of rainbow trout (Oncorhynchus mikyss) affected by the bacterial cold water disease (BCDW) with asymptomatic trout skin samples from fish farms in Lake Titicaca (Puno, Peru) and identify the presence of Flavobacterium psychrophilum by conventional PCR. Thirteen trout with skin lesions and seven without lesions, with sizes greater than 25 cm and average weight of 350 g were used. Conventional PCR was performed for the identification of Flavobacterium psychrophilum, using primers directed to the 16S rRNA region. Molecularly, both groups of animals were positive for the presence of F. psycrophilum. It can be concluded that this pathogen is present in the southern part of the country.
Los autores agradecen a FONDECYT-CONCYTEC por el apoyo financiero brindado al programa de Maestría en Sanidad Acuícola de la UPCH (Convenio de Gestión N.° 230-2015 FONDECYT–De-PROMOCIÓN 2), que permitió los análisis y la capacitación técnica de los investigadores para la ejecución del estudio. Del mismo modo, se agradece a los piscicultores y tesistas que colaboraron durante la recolección de muestras. Carlos Távara también agradece a Vitapro S.A. por el compromiso con la investigación en la acuicultura del Perú, siendo parte del soporte financiero del presente trabajo
Peer reviewed
histology, Histology, PCR, Oncorhynchus mikyss, Flavobacterium psychrophilum, histología
histology, Histology, PCR, Oncorhynchus mikyss, Flavobacterium psychrophilum, histología
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