Triploidy can be induced by retention of the 2nd polar body by temperature or pressure shocks. Induced triploidy is used in the aquaculture of some fish and mollusks to increase growth and avoid the problems associated with sexual maturation, since induced triploids are sterile. Most research on the consequences of induced triploidy has been focused on survival, growth, and reproductive physiology. However, less is known on the epigenetic regulation of gene expression and the limited literature reveals contrasting results. Thus, no differences were detected between diploid and triploid brown trout (Salmo trutta). In triploid oysters (Crassostrea gigas), different levels of DNA methylation were observed related to their fertility status. When compared to diploids, and regardless of sex, triploid oysters had more hypomethylated regions if they were infertile and more hypermethylated if they were fertile. In allotriploid cyprinids, DNA methylation was involved in dosage compensation, resulting in similar gene expression levels than diploids. Finally, DNA methylation was suggested to contribute to autopoliploidy–mediated speciation in marbled crayfish (Procambarus virginalis). However, no information is available on the effects of induced triploidy on DNA methylation patterns during early development in fish, which was the goal of this study

12th International Symposium on Reproductive Physiology of Fish (ISRPF), 15-19 May 2023, Crete, Greece

Funded by Ministry of Science and Innovation grant ‘Epipure’ (PID2019-108888RB-I00) to FP