The Production of Recombinant African Swine Fever Virus Lv17/WB/Rie1 Strains and Their In Vitro and In Vivo Characterizations

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 17 Dec 2023 Spain English Publisher:MDPI AGJournal:Vaccines, volume 11, page 1,860 (eissn: 2076-393X,

Copyright policy )Funded by:EC | VACDIVA

Authors: Stefano Petrini; Cecilia Righi; István Mészáros; Federica D’Errico; Vivien Tamás; Michela Pela; Ferenc Olasz; +6 Authors

doi: 10.3390/vaccines11121860

pmid: 38140263

pmc: PMC10748256

handle: 10261/348157

The Production of Recombinant African Swine Fever Virus Lv17/WB/Rie1 Strains and Their In Vitro and In Vivo Characterizations

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Abstract

Lv17/WB/Rie1-Δ24 was produced via illegitimate recombination mediated by low-dilution serial passage in the Cos7 cell line and isolated on PAM cell culture. The virus contains a huge ~26.4 Kb deletion in the left end of its genome. Lv17/WB/Rie1-ΔCD-ΔGL was generated via homologous recombination, crossing two ASFV strains (Lv17/WB/Rie1-ΔCD and Lv17/WB/Rie1-ΔGL containing eGFP and mCherry markers) during PAM co-infection. The presence of unique parental markers in the Lv17/WB/Rie1-ΔCD-ΔGL genome indicates at least two recombination events during the crossing, suggesting that homologous recombination is a relatively frequent event in the ASFV genome during replication in PAM. Pigs infected with Lv17/WB/Rie1-Δ24 and Lv17/WB/Rie1/ΔCD-ΔGL strains have shown mild clinical signs despite that ASFV could not be detected in their sera until a challenge infection with the Armenia/07 ASFV strain. The two viruses were not able to induce protective immunity in pigs against a virulent Armenia/07 challenge.

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Spain

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Keywords

illegitimate recombination, Live-attenuated vaccine, R, pigs, live-attenuated vaccine, homologous recombination, Illegitimate recombination, Article, Medicine, Pigs, ASFV, African swine fever, Homologous recombination

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