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A simplified courtship conditioning protocol to test learning and memory in Drosophila

Authors: Gil Martí, Beatriz; Barredo, Celia G.; Pina-Flores, Sara; Poza-Rodriguez, A.; Treves, G.; Rodríguez-Navas, Carmen; Camacho, L.; +5 Authors

A simplified courtship conditioning protocol to test learning and memory in Drosophila

Abstract

In Drosophila, a male that has previously been sexually rejected reduces its courtship behavior when confronted again with an unreceptive female. This reduced courting time reflects a memory formation process. Here, we describe a simplified protocol to perform the courtship conditioning assay for assessing the reduced courting time, using regular lab equipment and handmade tools. Every step of the procedure, from raising flies and training to testing and quantification of this memory-related behavior, can be implemented in any practice laboratory.

Country
Spain
Keywords

Male, Behavior, Science (General), Courtship, Q1-390, Model Organisms, Drosophila melanogaster, Memory, Protocol, Animals, Learning, Female, Drosophila, Neuroscience

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
views
OpenAIRE UsageCountsViews provided by UsageCounts
downloads
OpenAIRE UsageCountsDownloads provided by UsageCounts
8
Top 10%
Average
Top 10%
44
54
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gold