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Cytotherapy
Article . 2023 . Peer-reviewed
License: CC BY NC ND
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DIGITAL.CSIC
Article . 2024 . Peer-reviewed
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Cytotherapy
Article . 2023
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Three-dimensional cell culture of chimeric antigen receptor T cells originated from peripheral blood mononuclear cells towards cellular therapies

Authors: Eduardo Pérez del Río; Macarena Román Alonso; Irene Rius; Fabião Santos; Miquel Castellote-Borrell; Jaume Veciana; Imma Ratera; +2 Authors

Three-dimensional cell culture of chimeric antigen receptor T cells originated from peripheral blood mononuclear cells towards cellular therapies

Abstract

With the objective of improving the ex vivo production of therapeutic chimeric antigen receptor (CAR) T cells, we explored the addition of three-dimensional (3D) polystyrene scaffolds to standard suspension cell cultures.We aimed to mimic the structural support given by the lymph nodes during in vivo lymphocyte expansion.We observed an increase in cell proliferation compared with standard suspension systems as well as an enhanced cytotoxicity toward cancer cells. Moreover, we directly obtained the CAR T cells from peripheral blood mononuclear cells, thus minimizing the ex vivo manipulation of the therapeutic cells and opening the way to synergies among different cell populations.We propose the use of commercially available 3D polystyrene systems to improve the current immune cell cultures and resulting cell products for emerging cellular (immuno)therapies.

Countries
Spain, Spain
Keywords

3D cell culture, CAR T cells, Receptors, Chimeric Antigen, COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::proteínas de membranas::receptores de superficie celular::receptores artificiales::receptores de antígenos quiméricos, ANATOMÍA::células::células sanguíneas::leucocitos::leucocitos mononucleares::linfocitos::linfocitos T, Càncer - Tractament, CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, Artificial::Receptors, Chimeric Antigen, CHEMICALS AND DRUGS::Biomedical and Dental Materials::Polymers::Plastics::Polystyrenes, 3D scaffolds, T-Lymphocytes, TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::diagnóstico::técnicas y procedimientos diagnósticos::técnicas de laboratorio clínico::técnicas citológicas::técnicas de cultivo celular, Cèl·lules T - Ús terapèutic, Adoptive cell therapy, Cell expansion, Poliestirè, ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Cytological Techniques::Cell Culture Techniques, Peripheral blood mononuclear cells, COMPUESTOS QUÍMICOS Y DROGAS::materiales biomédicos y dentales::polímeros::plásticos::poliestirenos, Leukocytes, Mononuclear, Polystyrenes, Cell Culture Techniques, Three Dimensional, Cultiu cel·lular, ANATOMY::Cells::Blood Cells::Leukocytes::Leukocytes, Mononuclear::Lymphocytes::T-Lymphocytes

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selected citations
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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