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handle: 10902/1141 , 10261/34218
Esta invención presenta secuencias, vectores y microorganismos GTF que están constituidas o comprenden el gen gtf de P. damnosus 2.6 que codifica una glicosiltransferasa localizada en la membrana y que permite la producción de exopolisacáridos del tipo β-glucano por sobreexpresión de dicho gen recombinante. El hecho de que sea un único gen el implicado en la síntesis de este enzima facilita la optimización de las condiciones de expresión que permitirán la producción de β-glucanos a escala industrial. Estos polímeros, que pueden ser utilizados como aditivos en numerosos alimentos, poseen además propiedades beneficiosas para la salud humana. Por otro lado, estos microorganismos GTF transformados pueden ser utilizado en procesos de fermentación de alimentos como productos lácteos, bebidas alcohólicas y avena.
The invention relates to GTF micro-organisms, vectors and sequences which are formed by or comprise gene gtf de P.damnosus 2.6 which encodes a glycosyltransferase that is located in the membrane and which enables the production of exopolysaccharides of the β-glucan type by overexpression of said recombinant gene. The fact that only one gene is involved in the synthesis of the aforementioned enzyme facilitates the optimisation of the expression conditions which enable the production of β-glucans on an industrial scale. Said polymers, which can be used as additives in numerous foods, also have properties that are beneficial to human health. Moreover, said transformed GTF micro-organisms can be used in processes for the fermentation of foods, such as dairy products, alcoholic drinks and oats.
Nº Pub. Solicitud Internacional: WO2006/032718A1 (30.03.2006)
Solicitud Internacional: PCT/ES2005/070127 (08.09.2005)
Nº Pub. Solicitud Europea: EP1847598A1 (24.10.2007)
Solicitud Europea: 05784933 (08.09.2005)
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